The only hurdle I see with you moving to the airlines is your lack of a college degree. The merits of whether they should or should not be required can be debated elsewhere, fact of the matter is most major/legacy carriers require it or at least the VAST majority of their new hires will have a degree. Many regional carriers would prefer you to have a degree.

Beyond that, 121 is a totally different world than you are use to, there are good and bad things depending on what type of flying you like to do and what sort of work schedule you like. Good luck down the road.