Government positions seem more concerned with a specific number of hours in the last six or twelve months, but some companies do ask.

If you're going for an airline position, most will ask if you're current; as you've noted, legally current (IPC, flight review), as well as technically current and proficient, is what they're after. An interview that features a simulator check will verify basic skills.

In most cases, if I evaluate a pilot, I know most of what I need to know before we ever get to an airplane or a simulator. With this in mind, be current on all things job-related. Be able to answer ATP-level questions and know instrument charts, airspace, regulations, and so forth, fluidly. Flying is much like riding a bike (albeit more difficult to place baseball cards in the spokes, as they say), but there's a level of familiarity and ease in talking the talk, as well as walking the walk, which will come through in an interview. Read up and brush up to be ready.

Regionals hire very inexperienced pilots. You don't need to be the ace fo the base, but you do need to be competent and ready, and as you noted, it's largely about your comfort level. Someone else can sign you off for a proficiency check or flight review, but only you know when the rust is sufficiently scrubbed.