Hopefully a little additive can help with the freeze point. Jet fuel has a BTU (energy release) of about 132000-135000 per gallon. Biofuel is in the 130000-132000 range, so the energy difference is fairly small. The emissions reduction would be in the 60-80% range, according to Boeing.

Jet fuel is about 6.84 pounds per gallon, give or take due to the specific gravity of each batch made. Biofuel is 7.5 pounds per gallon.

Kudos to CAL for doing this flight.