well strictly speaking, 110 and 130 seat rj's are just right out (i hope someone gets the monty pyton joke)
before we get to saying where the line needs to be. i think that all "regional" pilots should understand that unless they REALLY like the lifestyle they should stick it out at there company.
for me, i believe that any single legs over 300 NM should be flown by mainline aircraft and aircrew. anything under that COULD be flown by a "regional" carrier. i think the shift that was made was from props to jets, (in the "regional" world really 3825'ed everything up for everyone.